Replication of polyoma DNA in isolated nuclei. V. Complementation of in vitro DNA replication

The replication of polyoma DNA.

Polyoma DNA synthesis in isolated nuclei: evidence for defective replication forks.

Nuclei, isolated from polyoma virus-infected mouse 3T6 cells, were incubated under condtions suitable for polyoma DNA synthesis. By using electrom microscopy and standard regression statistics, it was shown that replication is mainly unidirectional in a large number of molecules, indicating the presence of inactive replication forks. The replication forks were inactivated randomly, and the defe...

Polyoma virus DNA replication is semi-discontinuous.

In marked contrast to simian virus 40 (SV40), polyoma virus (PyV) has been reported to replicate discontinuously on both arms of replication forks. In an effort to clarify the relationship between the mechanisms of DNA replication in these closely related viruses, the distribution of RNA-primed DNA chains at replication forks was examined concurrently in PyV and SV40 replicating DNA purified fr...

Replication of DNA by nuclei isolated from soybean suspension cultures.

DNA replication was studied in nuclei isolated from soybean cells grown in suspension culture. The isolation procedure involved the preparation of protoplasts, their lysis with a nonionic detergent and purification of nuclei. These nuclei synthesized low molecular weight DNA and joined these fragments into DNA of intermediate molecular weight. The characteristics of replication in isolated nucl...

DNA REPLICATION AND SYNTHESIS OF DNABINDING PROTEINS IN THE CHLOROPLASTS OF A CALLUS CULTURE

Continuous labelling of callus with H-thymidine results in intermittent peaks of H-DNA per chloroplast, showing synchrony of division. The increase in H-DNA could be due to several replication rounds, and the drop to successive plastid divisions without intervening DNA synthesis. The level of DNA-binding proteins in the chloroplast parallels the peaks of plastidal DNA synthesis; such pro...